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Scientific Devices

Tewitro® TW 24

Transepidermal Waterloss Measurement in Cultured Skin Samples

TWQuadratThe Tewitro® TW 24 is the only device to measure the water evaporation from cultured tissue sets (wells in a plate with medium) in up to 24 wells simultaneously with the worldwide most used open chamber measurement of the Tewameter®.

 

 

 


 

 

Fields of Application

The Tewitro® Measurement Principle

The Software

Typical Application Examples

What do you need to get started?

Technical Data

 

Fields of Application

For each product to be applied to the skin, safety measurements are indispensable. The use of cultured cells sets for long-term safety tests is a quick and easy method to avoid using animals or volunteers. It is:

  • the only way to study skin permeability and dermal absorption necessary for safety and efficacy testing
  • well established in different guidelines around the globe and approved by institutions such as ECVAM (European Centre for the Validation of Alternatives to Animal Testing)

Whenever the barrier is damaged, the evaporation rate will increase immediately. Also for claims related to barrier improvement/enhancing, tests on cultured cells are needed, as products meant to keep the barrier intact or to even improve it, can be detected by a stable Transepidermal Water Loss (TEWL).

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The Tewitro® Measurement Principle

Fully equipped, the Tewitro® TW 24 can be used in 24 wells plates (6x4). The probe features 6 slots with 4 measurement inlets each, but is also available as TWinletsingle rows of 1 slot with 4 measuring inlets (respectively for 8, 12, 16 or 20 inlets), depending on the used well plate size.
Each measurement inlet features two sensor pairs constantly measuring temperature and relative humidity, thus measuring in an indirect way the density gradient of the water evaporation from the surface of the bottom of the well. This gradient equals the Transepidermal Water Loss typically measured on the in vivo skin surface in g/h/m². This "open chamber" TEWL measurement method is the world-wide most-used method to quantify the barrier function of the skin. Hundreds of publication prove this fact. The Tewameter® is even used on the ISS (International Space Station) to monitor the astronaut's skin during long-term exposure in space.

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The Software

  • The convenient and organized software offers 24 TEWL-values (one for each measurement inlet), with two different adjustable averages and their respective standard deviations as well as the possibility of displaying and saving the RH and temperature value for the upper TMSWcloseupand lower sensor pair for each measurement inlet (2 x 24) separately.
  • Graphical view for each measurement inlet with one click.
  • Measurements from a couple of seconds up to 24 h long-term studies are possible.
  • Automatic stop conditions for the measurement (stability of the measurement or time)
  • Easy & quick checkcalibration and offset.
  • If the Ambient Condition Sensor probe is connected, its values are constantly recorded during the measurement and saved with the results.

    tempsensor



TWtable viewTWcurve view

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Typical Application Examples

On pre-cultured cells before the product(s) to be tested are applied:twproduct appl
Constant measurement of the evaporating water which is typical for the wells when coming out of the incubator until a normal TEWL value (for incubated cell cultures) is reached. When the probe starts to measure, the TEWL is very high but constantly decreasing. As soon as it has reached a stable plateau, it indicates that the cell cultures are now ready for the application of the product. At the same time, the recorded TEWL is a control value (before application).
Benefits:

  • The probe can constantly measure over longer periods (only possible with open chamber measurements).
  • The software can indicate that the TEWL has reached a stable threshold value.
  • You can be sure to apply the tested products in repeated tests to cell cultures under the same conditions.
  • Control value of all wells used is recorded before application, so that the later measured effects can be surely attributed to the product.


Measuring the actual effect of the barrier function of applied products (safety & efficacy):

When the well-plate cTW2omes out of the incubator, the TEWL measurement can again be used to monitor when the surplus humidity from the incubator has left the samples and the real measurement time starts. Depending on the nature of the applied products the barrier of the cultured cells is more or less intact. When the barrier has been damaged, particles of the cells can be found (microbiologically) in the surrounding medium. However, the degree of damage or barrier improvement can very accurately be indicated by the monitored TEWL- values. The higher they stay, the less intact the barrier.

Additional Benefits:

  • The micro climate in the wells, once they are out of the incubator, is constantly changing. After leaving the incubator, the cultured cells also dry quickly and shrink soon. Therefore, to get meaningful results, it is important to measure all wells at the same time/same conditions and not miss the correct measurement point. If you perform single measurements in each well one after the other, the values will never be comparable. For 24 wells such a procedure would take way too long to get reproducible results.
  • Extremely time- (and manpower-) saving and economic.
  • The open chamber TEWL measurement do not inhibit the normal evaporation rate and does not cause occlusion.

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What do you need to get started?

The Tewitro® TW 24 can measure in the well plates of cultured tissue. Until now we have experience with the following readily TW close upon the market available models:


For other models, please check with us by providing a sample. The probe can be custom fitted to many different systems (ask for a price offer).
The Tewitro® TW 24 probe connects to the computer via a Multi Probe Adapter system (MPA).
For the measurement of TEWL, the plate with the wells and the measurement probe has to be heated to skin temperature. The laboratory condition is rather cool (20-22°C), in this condition differences in TEWL are barely visible. As soon as the system is heated to skin temperature, the differences become immediately obvious. We recommend to use a heating plate with a temperature around 33 °C.

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Brochure Download

Technical Data

Probe only:

Dimensions: 113 (W) x 170 (L) x 32 (H) mm, weight: 300 g, cable length: approx. 1.20 m, frame material: anodized aluminum (AlMg 3), power consumption: max. 12 V
In full equipment: 24 sensor pairs (48 single sensors)
Measurement range: Temperature: 0 - 50° C, resolution: typ. 0.015 °C, RH: 0% - 100% RH, resolution: typ. 0.01 % RH, TEWL: 0 to 320 g/h/m²
Measurement uncertainty: for 20-50°C and RH ≤ 80%: typ. ± 1.5% RH, max. ± 2% RH, typ. ± 0.1°C, max. ± 0.3°C
Operating conditions: T: 5-40° C RH: 30-70 %

Technical changes may be made without prior notice.

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